Kin-Yiu Wong
Hong Gu
Shuming Zhang
Joseph A. Piccirilli
Michael E. Harris
Darrin M. York

Abstract

The primary and secondary kinetic isotope effects for a model compound which represents RNA cleavage transesterification were calculated and compared with experimental measurements. Based on the good agreement between theory and experiments, the energy profile, reaction pathway, and two distinct transition states for the reactions of the model compound and two thio-substituted analogues were characterized.