Chemical Communications vol. 52 p. 4462-4465 DOI: 10.1039/C5CC10212J
PMID/PMCID: PMC4916458 Published: 2016-01-28
Solvent D2O and 18O kinetic isotope effects on RNA 2'-O-transphosphorylation catalyzed by Zn2+ demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.