Isotope effect analyses provide evidence for an altered transition state for RNA 2'-O-transphosphorylation catalyzed by Zn2+

Chemical Communications vol. 52  p. 4462-4465  DOI: 10.1039/C5CC10212J
PMID/PMCID: PMC4916458 Published: 2016-01-28 

Shuming Zhang, Hong Gu, Haoyuan Chen, Emily Strong, Edward W. Ollie, Daniel L. Kellerman, Danni Liang, Masaru Miyagi, Vernon E. Anderson, Joseph A. Piccirilli, Darrin M. York [ ] , Michael E. Harris

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Solvent D2O and 18O kinetic isotope effects on RNA 2'-O-transphosphorylation catalyzed by Zn2+ demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.