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Isotope effect analyses provide evidence for an altered transition state for RNA 2'-O-transphosphorylation catalyzed by Zn2+

Isotope effect analyses provide evidence for an altered transition state for RNA 2'-O-transphosphorylation catalyzed by Zn2+

Chemical Communications vol. 52  p. 4462-4465  DOI: 10.1039/C5CC10212J  
PMID/PMCID: PMC4916458 Published: 2016-01-28 

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Shuming Zhang
Hong Gu
Haoyuan Chen
Emily Strong
Edward W. Ollie
Daniel L. Kellerman
Danni Liang
Masaru Miyagi
Vernon E. Anderson
Joseph A. Piccirilli
Darrin M. York
Michael E. Harris

Abstract

Solvent D2O and 18O kinetic isotope effects on RNA 2'-O-transphosphorylation catalyzed by Zn2+ demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.